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2005  (Bio-Techne corporation)


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    Bio-Techne corporation 2005
    2005, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 4968 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2005/product/Bio-Techne corporation
    Average 99 stars, based on 4968 article reviews
    2005 - by Bioz Stars, 2026-06
    99/100 stars

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    Effects of p‐ Cresol on <t>DITNC1</t> astrocytes. (A) Experimental workflow: (B–E) Representative images of astroglial cells incubated with vehicle (Ctrl), and concentrations of 50, 150, or 300 μM of p ‐Cresol for 24 h following CellROX (green) staining. Cell nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F) Quantitative results of the DAPI + astrocyte cell count 24 h after stimulation with either vehicle or different p ‐Cresol concentrations. (G) Effects of p‐ Cresol on oxidative stress in DITNC1 cells measured using CellROX staining. Results are expressed as mean ± SEM values. Statistical analyses were conducted using two‐way ANOVA with Tukey's multiple comparison tests: * p < 0.05 vs. Ctrl, *** p < 0.001 vs. Ctrl, $$ p < 0.01 vs. p‐ Cresol 50 μM.
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    2005  (Bio-Techne corporation)
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    Effects of p‐ Cresol on <t>DITNC1</t> astrocytes. (A) Experimental workflow: (B–E) Representative images of astroglial cells incubated with vehicle (Ctrl), and concentrations of 50, 150, or 300 μM of p ‐Cresol for 24 h following CellROX (green) staining. Cell nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F) Quantitative results of the DAPI + astrocyte cell count 24 h after stimulation with either vehicle or different p ‐Cresol concentrations. (G) Effects of p‐ Cresol on oxidative stress in DITNC1 cells measured using CellROX staining. Results are expressed as mean ± SEM values. Statistical analyses were conducted using two‐way ANOVA with Tukey's multiple comparison tests: * p < 0.05 vs. Ctrl, *** p < 0.001 vs. Ctrl, $$ p < 0.01 vs. p‐ Cresol 50 μM.
    2005, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    wei 2005  (Mallinckrodt)
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    Effects of p‐ Cresol on <t>DITNC1</t> astrocytes. (A) Experimental workflow: (B–E) Representative images of astroglial cells incubated with vehicle (Ctrl), and concentrations of 50, 150, or 300 μM of p ‐Cresol for 24 h following CellROX (green) staining. Cell nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F) Quantitative results of the DAPI + astrocyte cell count 24 h after stimulation with either vehicle or different p ‐Cresol concentrations. (G) Effects of p‐ Cresol on oxidative stress in DITNC1 cells measured using CellROX staining. Results are expressed as mean ± SEM values. Statistical analyses were conducted using two‐way ANOVA with Tukey's multiple comparison tests: * p < 0.05 vs. Ctrl, *** p < 0.001 vs. Ctrl, $$ p < 0.01 vs. p‐ Cresol 50 μM.
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    Image Search Results


    Effects of p‐ Cresol on DITNC1 astrocytes. (A) Experimental workflow: (B–E) Representative images of astroglial cells incubated with vehicle (Ctrl), and concentrations of 50, 150, or 300 μM of p ‐Cresol for 24 h following CellROX (green) staining. Cell nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F) Quantitative results of the DAPI + astrocyte cell count 24 h after stimulation with either vehicle or different p ‐Cresol concentrations. (G) Effects of p‐ Cresol on oxidative stress in DITNC1 cells measured using CellROX staining. Results are expressed as mean ± SEM values. Statistical analyses were conducted using two‐way ANOVA with Tukey's multiple comparison tests: * p < 0.05 vs. Ctrl, *** p < 0.001 vs. Ctrl, $$ p < 0.01 vs. p‐ Cresol 50 μM.

    Journal: Journal of Neurochemistry

    Article Title: Unveiling the Molecular Mechanism of Intestinal Metabolite para ‐Cresol in Modulating Neuroinflammation and Synaptic Dysfunction: Implications for Autism Spectrum Disorder

    doi: 10.1111/jnc.70457

    Figure Lengend Snippet: Effects of p‐ Cresol on DITNC1 astrocytes. (A) Experimental workflow: (B–E) Representative images of astroglial cells incubated with vehicle (Ctrl), and concentrations of 50, 150, or 300 μM of p ‐Cresol for 24 h following CellROX (green) staining. Cell nuclei were counterstained with DAPI (blue). Scale bar = 20 μm. (F) Quantitative results of the DAPI + astrocyte cell count 24 h after stimulation with either vehicle or different p ‐Cresol concentrations. (G) Effects of p‐ Cresol on oxidative stress in DITNC1 cells measured using CellROX staining. Results are expressed as mean ± SEM values. Statistical analyses were conducted using two‐way ANOVA with Tukey's multiple comparison tests: * p < 0.05 vs. Ctrl, *** p < 0.001 vs. Ctrl, $$ p < 0.01 vs. p‐ Cresol 50 μM.

    Article Snippet: DITNC1 (RRID:CVCL_0247, CRL‐2005, ATCC) rat astrocyte cells were cultured in Dulbecco's modified eagle medium (DMEM) (#D6546, Sigma) supplemented with 10% fetal bovine serum (FBS) (#2473339RP, Gibco), 2% L‐glutamine (#G7513, Sigma), and 1% penicillin/streptomycin (Pen/Strep) (P4333, Sigma).

    Techniques: Incubation, Staining, Cell Characterization, Comparison

    Effects of p ‐Cresol on gene expression of DITNC1 astrocytes. DITNC1 cells were treated with vehicle, 50 or 150 μM of p‐ Cresol for 24 h, then the total RNA was extracted, and qPCR was conducted. (A) Data are presented as fold change normalized over the Ctrl group mean value and calibrated over GAPDH/APOE housekeeping genes. The experimental conditions were tested in triplicate, and data were expressed as mean ± SEM values and analyzed by one‐way ANOVA (B). The colorimetric scale was used to show up‐ (green) or down‐ (red) regulated genes; * p < 0.05; *** p < 0.001; $$ p < 0.01 vs. p‐ Cresol 50 μM; #0.10 > p > 0.05.

    Journal: Journal of Neurochemistry

    Article Title: Unveiling the Molecular Mechanism of Intestinal Metabolite para ‐Cresol in Modulating Neuroinflammation and Synaptic Dysfunction: Implications for Autism Spectrum Disorder

    doi: 10.1111/jnc.70457

    Figure Lengend Snippet: Effects of p ‐Cresol on gene expression of DITNC1 astrocytes. DITNC1 cells were treated with vehicle, 50 or 150 μM of p‐ Cresol for 24 h, then the total RNA was extracted, and qPCR was conducted. (A) Data are presented as fold change normalized over the Ctrl group mean value and calibrated over GAPDH/APOE housekeeping genes. The experimental conditions were tested in triplicate, and data were expressed as mean ± SEM values and analyzed by one‐way ANOVA (B). The colorimetric scale was used to show up‐ (green) or down‐ (red) regulated genes; * p < 0.05; *** p < 0.001; $$ p < 0.01 vs. p‐ Cresol 50 μM; #0.10 > p > 0.05.

    Article Snippet: DITNC1 (RRID:CVCL_0247, CRL‐2005, ATCC) rat astrocyte cells were cultured in Dulbecco's modified eagle medium (DMEM) (#D6546, Sigma) supplemented with 10% fetal bovine serum (FBS) (#2473339RP, Gibco), 2% L‐glutamine (#G7513, Sigma), and 1% penicillin/streptomycin (Pen/Strep) (P4333, Sigma).

    Techniques: Gene Expression